Chromogenix

Each vial contains:

• Lyophilized human Antithrombin 25 – 30 lU
• Sodium Chloride 11 mg

Each vial contains chromogenic substrate S-2238™ 25 mg and mannitol 120 mg as a bulking agent. Stability: Substance: Stable until expiry date if stored at 2-8°C. Avoid exposure to light. The substance is hygroscopic and should be stored dry. Solution: 1 mmol/L in H2O is stable for more than 6 months at 2-8°C.

Chemical name: H-D-Phenylalanyl-L-pipecolyl-Larginine-p-nitroaniline dihydrochloride.

pFormula: H-D-Phe-Pip-Arg-pNA·2 HCl

Mol. wt.: 625.6

e316 nm:1.27 . 104 mol-1 . L . cm-1

Solubility: > 10 mmol/L in H2O

Suitable stock solution: 1-2 mmol/L in H2O.

Each vial contains the chromogenic substrate S-2765™, 25 mg and mannitol 60 mg as a bulking agent.

Stability: Lyophilized substance: stable at 25°C until expiry date printed on the label. The substance is hygroscopic and should be stored in a dry place.

Solution: 2 mmol/L in H2O is stable for six months at 2 to 8°C. Suitable stock solution: 1-2 mmol/L in H2O.

Chemical name:N-a-Benzyloxycarbonyl-Darginyl-L-glycyl-L-arginine-pnitroaniline-dihydrochloride

Formula:N-a-Z-D-Arg-Gly-Arg-pNA·2HCl

Mol. wt.: 714.6

e316 nm:1.27 . 104 mol-1 . L . cm-1

Solubility: > 10 mmol/L in H2O

Suitable stock solution: 1-2 mmol/L in H2O.

Each vial contains chromogenic substrate S-2251™, 25 mg and mannitol 60 mg as a bulking agent. Stability: Substance: Stable until expiry date if stored at 2-8°C. Avoid exposure to light. The substance is hygroscopic and should be stored dry. Solution: 3 mmol/L in H2O is stable for at least 6 months at 2-8°C. Contamination by microorganisms may cause hydrolysis.

Chemical name:H-D-Valyl-L-leucyl-L-lysinep-Nitroaniline dihydrochloride

Formula:H-D-Val-Leu-Lys-pNA · 2HCl

Mol. wt.: 551.6

Each vial contains chromogenic substrate S-2302™ 25 mg and mannitol 60 mg as a bulking agent. Stability: Substance: Stable until expiry date if stored at 2-8°C. Avoid exposure to light. The substance is hygroscopic and should be stored dry. Solution: 4 mmol/l in H2O is stable for more than 6 months at 2-8°C.

Chemical name:H-D-Prolyl-L-phenylalanyl-Larginine-p-nitroaniline dihydrochloride.

Formula:H-D-Pro-Phe-Arg-pNA·2HCl

Mol. wt.:611.6

Each vial contains chromogenic substrate S-2222™ 25 mg and mannitol 120 mg as a bulking agent.

Molecular wt: 741.3

Chemical name:N-Benzoyl-L-isoleucyl-L-glutamyl-glycyl-Larginine-p-nitroaniline hydrochloride and its methyl ester

Formula:Bz-Ile-Glu(g-OR)-Gly-Arg-pNA·HCl R=H (50%) and R=CH3 (50%)

Each vial contains chromogenic substrate S-2288™, 25 mg and mannitol 40 mg as a bulking agent. Stability: Substance: Stable until expiry date if stored at 2-8°C. Avoid exposure to light. The substance is hygroscopic and should be stored in a dry place. Solution: 10 mmol/L in H2O is stable for more than two months at 2-8°C.

Chemical name:H-D-Isoleucyl-L-prolyl-L-arginine-pnitroaniline dihydrochloride.

Formula:H-D-lle-Pro-Arg-pNA · 2HCl

Mol. wt: 577.6

Each vial contains the chromogenic substrate S-2403™ 25 mg and mannitol 60 mg as a bulking agent. Stability: Substance: Stable until expiry date if stored at 2-8°C. Avoid exposure to light. The substance is hygroscopic and should be stored dry. Solution: 7.5 mmol/L in H2O is stable for at least 6 months at 2 to 8°C.

Chemical name:L-Pyroglutamyl-L-Phenylalanyl-L-Lysine-p-Nitroaniline hydrochloride.

Mol. wt: 561.0

Reagents, Packaging, Storage, Stability The sealed reagents are stable at 2-8°C until the expiry date printed on the label.

S-2732™, 15 mg,2 vials. Chromogenic substrate, Suc-Ile-Glu(-pip)-Gly-Arg-pNA·HCl lyophilized with detergent and mannitol as bulking agent. Stability after reconstitution: 3 months at +2-8°C in the original vial. Avoid exposure to light. Discard the substrate solution if it appears yellow. Avoid contamination by microorganisms.

Factor Xa, 35 nkat,2 vials. Lyophilized bovine FXa containing Tris buffer, EDTA, NaCl, dextran sulfate and bovine serum albumin. Stability after reconstitution: 3 months at +2-8°C in the original vial.

Reagents, Packaging, Storage, Stability, and Microplate/Test Tube Preparation

• S-2222™, 1 vial. Chromogenic substrate (Bz-IIe-Glu-(g-OR)-Gly-Arg-pNA·HCl) 15 mg with mannitol added as a bulking agent. Reconstitute with 20 mL sterile water to obtain a concentration of 1 mmol/l. The solution is stable for 6 months at 2-8°C.
• Factor Xa, 1 vial. Bovine Factor Xa 71 nkat. Reconstitute with 10 mL sterile water. The reconstituted Factor Xa is stable for 1 month at 2-8°C or 6 months at -20°C or below.
• Buffer, stock solution, 1 vial. Tris 0.5 mol/l, pH 8.4, 10 ml. An opened vial of stock solution is stable for 2 months at 2-8°C. Before use dilute accordingly: 1 volume of stock solution with 9 volumes of sterile water.
• Antithrombin, 1 vial. Lyophilized human Antithrombin, 10 IU. Reconstitute with 10 mL sterile water to obtain a concentration of 1 IU/ml. The reconstituted Antithrombin is stable for 1 month at 2-8°C or 6 months at -20°C or below.
• Normal Plasma (human), 4 vials. Lyophilizied plasma. Reconstitute with 1.00 mL sterile water. The reconstituted plasma is stable for two weeks at 2-8°C or 1 month at -20°C or below.

When kept at 2-8°C the sealed reagents are stable until expiry date printed on the label. Avoid contamination by microorganisms in opened vials.

Reagents, Packaging, Storage, Stability, and Microplate/Test Tube Preparation

• S-2765™ 15.4 mg + I-2581, 1 vial. Chromogenic substrate (N-a-Z-D-Arg-Gly-Arg-pNA), 15.4 mg, synthetic thrombin inhibitor, 0.4 mg, and mannitol added as a bulking agent. Reconstitute with 12.0 mL of sterile water or NCCLS type II water, to obtain a concentration of 1.8 mmol/L. Stability after reconstitution: 3 months at 2-8°C.
• Factor IXa + factor X 9.2 IU, 1 vial. Lyophilized bovine factors IXa and X with bovine albumin added as a stabilizing agent. Reconstitute with 10.0 mL of sterile water or NCCLS type II water. Stability after reconstitution: 12 hours at 2-8°C. The solution can be stored frozen in aliquots at -20°C (or at lower temperature) for 3 months. Do not refreeze.
• CaCl2 6 ml, 1 vial. Calcium chloride solution, 0.025 mol/L Ready to use. Opened vial is stable 3 months at 2-8°C.
• Buffer, stock solution 20 mL,1 vial. 20 mL concentrated Tris buffer containing NaCl and BSA. Characteristics of tenfold diluted buffer: Tris 0.05 mol/L, pH 7.3, 10 mg/L Ciprofloxacin and 1.0% BSA. Dilute 1:10 (1+9) with sterile water or NCCLS type II water. Prepare a new buffer working solution each day. Once opened the buffer is stable 3 months at 2-8°C.
• Phospholipid 2 mL, 1 vial. Mixture of highly purified phospholipids and 10 mg/L Ciprofloxacin. Ready to use. When kept at 2-8°C the sealed reagents are stable until the expiry date printed on the label.

Reagents, Packaging, Storage, Stability, and Microplate/Test Tube Preparation

• S-2765™ 15.4 mg + I-2581,1 vial. Chromogenic substrate (N-a-Z-D-Arg-Gly-Arg-pNA), 15.4 mg, synthetic thrombin inhibitor, 0.4 mg, and mannitol added as a bulking agent. Reconstitute with 12.0 mL of sterile water or NCCLS type II water, to obtain a concentration of 2.7 mmol/L. Stability after reconstitution: 3 months at 2-8°C.
• Factor IXa + factor X 2.7 IU, 4 vials. Lyophilized bovine factors IXa and X with bovine albumin added as a stabilizing agent. Reconstitute with 3.0 mL of sterile water or NCCLS type II water. Stability after reconstitution: 12 hours at 2-8°C. The solution can be stored frozen in aliquots at -20°C (or at lower temperature) for 3 months. Do not refreeze.
• CaCl2 6 ml, 1 vial. Calcium chloride solution, 0.025 mol/L Ready to use. Opened vial is stable 3 months at 2-8°C.
• Buffer, stock solution 20 mL,1 vial. 20 mL concentrated Tris buffer containing NaCl and BSA. Characteristics of tenfold diluted buffer: Tris 0.05 mol/L, pH 7.3, 10 mg/L Ciprofloxacin and 1.0% BSA. Dilute 1:10 (1+9) with sterile water or NCCLS type II water. Prepare a new buffer working solution each day. Once opened the buffer.
• Phospholipid 2 mL,1 vial. Mixture of highly purified phospholipids and 10 mg/L Ciprofloxacin. Ready to use. Opened vial is stable for 3 months at 2-8°C. Shake gently before use. When kept at 2-8°C the sealed reagents are stable until the expiry date printed on the label. Contamination by microorganisms should be avoided once the vials are opened.